Assignment No. 2
BT301 – Introduction to
Biotechnology
Spring 2020
Total Marks: 10
Instructions:
● Make sure that you upload the solution file before due date.
No assignment will be acceptable through e-mail after the due date.
Formatting guidelines
● Use the font style “Times New Roman”
and font size “12”.
● Compose your document in MS-Word,
any file created in any format will not be accepted and marked zero.
● Use black and blue font colors only.
Solution
guidelines
● To solve this assignment, you should
have good command over Topics.
●
This is not a group assignment, it is an
individual assignment so be careful and avoid copying others’ work
●
Give the answer according to question only and
avoid irrelevant details.
Please note that your assignment will not be
graded if:
● It is submitted after due date
● The file you uploaded does not open
● The file you uploaded is copied from
someone else
● It is in some format other than .doc
● Cheating or copying of assignment
from internet,
ppt lessons or from each other is
strictly prohibited. The cheated or copied assignment will be marked ‘Zero’.
● Answers should be précised and
clear, with no useless material.
Assignment topic
1.
Write down five
similarities and five differences between Plasmid and Cosmid (10 Marks)
Note: Submit your solution through your lms account.
Solutions submitted through email will not be acceptable.
Assignment topic
1.
Write down five
similarities and five differences between Plasmid and Cosmid (10 Marks)
Solution
Plasmid
Plasmids are small circular pieces of DNA that replicate
independently from the host's chromosomal DNA. They are mainly found in
bacteria, but also exist naturally in archaea and eukaryotes such as yeast and
plants.
Cosmid.
Cosmid is a hybrid vector system. It is an artificial vector
constructed by combining the cos sequences of Lambda phage particles
and a plasmid.
Differences between plasmid and cosmid
The difference between plasmid and cosmid is that..
1.
the plasmid is a double-stranded, circular and closed extra-chromosomal DNA present in bacteria and archaea
while the cosmid is a hybrid vector system formed due to combining of the cos sequence of lambda phage and plasmid DNA of bacteria.
1.
the plasmid is a double-stranded, circular and closed extra-chromosomal DNA present in bacteria and archaea
while the cosmid is a hybrid vector system formed due to combining of the cos sequence of lambda phage and plasmid DNA of bacteria.
Similarities between plasmid and cosmid
1. Plasmid
and cosmid are vectors commonly used in recombinant DNA technology.
2. Both
are able to self-replicate.
3. They
have the origin of replication.
4. Furthermore,
they possess multiple cloning sites.
5. Also,
they contain antibiotic-resistant genes that are useful as markers.
6. Foreign
DNA can be inserted into both types and make recombinant molecules.
7. Easy
screening methods are available for both vectors.
8. Both
are useful to construct genomic libraries.
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